Coding
Part:BBa_K1500998:Design
Designed by: Kevin Yang Group: iGEM14_uchicago_IGSB (2014-10-17)
MutD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 174
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 46
Illegal AgeI site found at 535 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
NA
Source
PCR'd from DH5-alpha genomic sequence, with RBS.3 (medium) added as an overhang in front of the gene.